Fig. 1
Source data are provided in Additional File 1, Table S2. D Plasmid interference assay design. The putative PAMs are inserted in a plasmid upstream of a spacer identified in T. kivui CRISPR cluster, and functional PAM sequences are determined by antibiotic challenge. E Results from plasmid interference assay. Data represent three biological replicates (average ± standard deviation)
Investigation of the functionality of a type I-B CRISPR locus in T. kivui. A Genetic architecture of the CRISPR systems found in T. kivui. Squares represent spacer sequences; diamonds represent repeat regions. Spacer and repeat sequences are provided in Additional File 1, Table S1. B Sequence alignment of repeat regions from type I-B_4 CRISPR cluster of T. kivui identified with CRISPRCasFinder [34]. Non-conserved bases are highlighted in red (bold). The selected repeat sequence is highlighted with a box. CRISPRTarget [38] was used to find native protospacer targets in MGE databases. A representative example is shown, CRISPR4 S2 (spacer 2 from Type I-B_4 in blue, putative PAM sequence in yellow). C Putative PAM motif based on protospacer matches in CRISPRTarget [38]. Multiple sequence alignment of the reverse complement 3’ flank of protospacers visualized with Weblogo [53]).